Avaliação do extrato bruto de Artemisia vulgaris na resposta imunológica
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Abstract
A Artemisia vulgaris é uma das espécies do gênero Artemisia, gênero o qual possui grande história terapêutica e estudos. Suas partes aéreas são tradicionalmente consumidas pela população sob forma de infusão (chá), por acreditar que possui propriedades anti-helmíntica, antisséptica, antiespasmódica, anti-inflamatória e, até mesmo, como tônico para órgãos vitais. Este trabalho, teve como objetivo avaliar o extrato bruto (EB) da Artemisia vulgaris na resposta imunológica, com base na história terapêutica da planta e na etnofarmacologia . Para o desenvolvimento, foram utilizadas técnicas para obtenção e análise dos compostos do extrato por cromatografia líquida de alta eficiência (HPLC), sendo identificados como os principais compostos a artemisinina, e os ácidos clorogênico e cafeoilquínico. Posteriormente foi realizado o tratamento da linhagem celular RAW (macrófagos murinos) com o EB. As células foram tratadas com diferentes concentrações de extrato (de 0,25% a 2%), por um período de 24 horas, e estimuladas com lipopolissacarídio (LPS) bacteriano, com o intuito de se observar a produção de citocinas. Após a segunda incubação por 24 horas, o sobrenadante dessa linhagem foi recolhido, e a análise da produção de citocinas pró-inflamatórias, interleucina (IL)-6 e anti-inflamatórias, IL-4, foi feita por imunoensaio (teste de ELISA), avaliando, assim, a influência do extrato de A.vulgaris na resposta imunológica.
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