Decontamination protocol in the production of herbal medicines: a short communication

Conteúdo do artigo principal

Daniel Forjaz de Moraes
Juliana Vogt Ventramine Ivo Cabral
Lara Dhiovana da Silva
Larissa de Paiva Santos
Nicole Maria de Queiroz
Carlos Rocha Oliveira

Resumo

Background: The effectiveness and safety of natural products depend not only on the inherent properties of the plant, but also on careful preparation, which includes critical steps such as cleaning to remove impurities and washing to eliminate surface contaminants. Thus, the scientific literature emphasizes the significance of thorough cleaning in ensuring botanical purity, directly impacting the ultimate quality of the product. Methods: The study utilized the plant species Cordia verbenacea, Alternanthera brasiliana, and Lippia alba, cultivated in Tatuí - SP, subjected to a rigorous selection process, and processed through a washing and drying protocol. The washing involved a two-stage process, including immersion in clean water and a sodium hypochlorite solution. Microbiological analyses were conducted on washed and unwashed samples, including enumeration of viable colony-forming units (CFUs) and detection of pathogens. Moisture content was determined using the oven drying method, and ash content was assessed following the Brazilian Pharmacopeia guidelines. Results: Microbiological results indicate the absence of Salmonella sp. and Staphylococcus aureus in all analyzed plant drugs, regardless of washing and stabilization conditions. Pseudomonas aeruginosa was observed only in unwashed/stabilized Cordia verbenacea and Alternanthera brasiliana samples. Escherichia coli was identified in an unwashed/stabilized Lippia alba sample. The washing/stabilization process significantly reduced total bacterial and fungal counts, especially when exceeding the limit set by the Brazilian Pharmacopeia (103 CFU/g). Total ash content remained unchanged after the washing/stabilization process in the three plant species, staying within the Brazilian Pharmacopeia limits (14%). Moisture content did not differ statistically between washed/stabilized and unwashed/unstabilized plant species and met Brazilian Pharmacopeia specifications (8-14%). Conclusion: The washing/stabilization process effectively reduced microbial load in Lippia alba, Alternanthera brasilians, and Cordia verbenacea, with no significant changes in ash and moisture content. Preliminary analysis suggests that processing control ensures minimum quality conditions, enhancing overall safety for consumers.

Detalhes do artigo

Como Citar
1.
Forjaz de Moraes D, Vogt Ventramine Ivo Cabral J, Dhiovana da Silva L, de Paiva Santos L, Maria de Queiroz N, Oliveira CR. Decontamination protocol in the production of herbal medicines: a short communication. Braz. J. Nat. Sci [Internet]. 1º de dezembro de 2023 [citado 24º de fevereiro de 2024];5(1):E192202 - 1. Disponível em: https://bjns.com.br/index.php/BJNS/article/view/192
Seção
Artigo em fluxo contínuo
Biografia do Autor

Daniel Forjaz de Moraes, Autor da Própria Saúde Educacional, Barueri-SP, Brazil.

Autor da Própria Saúde Educacional, Barueri-SP, Brazil.

 

 

 

Juliana Vogt Ventramine Ivo Cabral, GAP Biotechnology, São José dos Campos-SP, Brazil.

GAP Biotechnology, São José dos Campos-SP, Brazil.

Lara Dhiovana da Silva, Anhembi Morumbi University, São José dos Campos-SP, Brazil

Anhembi Morumbi University, São José dos Campos-SP, Brazil

Larissa de Paiva Santos, Anhembi Morumbi University, São José dos Campos-SP, Brazil

Anhembi Morumbi University, São José dos Campos-SP, Brazil

Nicole Maria de Queiroz, Anhembi Morumbi University, São José dos Campos-SP, Brazil

Anhembi Morumbi University, São José dos Campos-SP, Brazil

Carlos Rocha Oliveira, Anhembi Morumbi University, São José dos Campos-SP, Brazil.

Anhembi Morumbi University, São José dos Campos-SP, Brazil.

Federal University of São Paulo (UNIFESP), Post-graduation Program in Biomedical Engineering, São José dos Campos-SP, Brazil.

GAP Biotechnology, São José dos Campos-SP, Brazil.

Referências

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Almeida, F. G., et al. (2019). Natural Stabilization Methods for Herbal Drugs: A Comprehensive Review. Phytotherapy Research, 26(4), 565-570.

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Costa, R. P. C., Guimarães, A. L. A., & Vieira, A. C. M. (2014). Avaliação da Qualidade de Amostras de Plantas Medicinais Comercializadas no Brasil. Revista Ciência Farmacêutica Básica e Aplicada, 35(3), 425-433. ISSN 1808-4532.

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Sonaglio, D., Ortega, G. G., Petrovick, P. R., & Bassani, V. L. (2003). Desenvolvimento tecnológico e produção de fitoterápicos. In: Simões, C. M. O., Schenkel, E. P., Gosmann, G., Mello, J. C. P., Mentz, L. A., & Petrovick, P. R. (Eds.), Farmacognosia: da planta ao medicamento (5ª ed.). Porto Alegre/Florianópolis: Editora da UFRGS/Editora da UFSC.

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