Phytochemical Screening, Characterization of Essential Oil and Antimicrobial Activity of Schinus molle (Anacardiaceae) Collected from Eastern Hararghe, Ethiopia
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Abstract
In the present study, antibacterial activity of leaf, stem bark and root bark extracts of Schinus molle (Anacardiaceae) was evaluated against two bacterial (Xanthomonas and Ranstonia) and two fungal (Aspergillusniger and Fusariumvert) species using agar diffusion method. The stem and root ethanol extracts of Schinusmolle exhibited relatively higher zone of inhibition (11.3mm) against Xanthomonas campestris, pv. Campestris. Higher zone of inhibition (10mm) was also observed by the ethanolic extracts of the root of Schinus molle against Fusarium verticillioides followed by the chloroform extracts of the root (8mm) against Aspergillus niger. Phytochemical analysis of leaf, stem bark and root bark extracts of Schinus molle revealed the presence of flavonoids, alkaloids, saponins, steroids, terpenoids, phenols, quinines, carbohydrates and proteins. The occurrence of these biologically active chemicals in the plants parts may justify their wide usage in traditional medicine. From GC-MS analysis, three terpenoids namely monoterpene (66.02%) as a major components, sesquiterpene hydrocarbons (13.63%) and oxygenated sesquterpenes (11.07%) were identified from the roots. The essential oils obtained from leaves have been also rich in oxygenated sesquterpenes (68.28%), followed by sesquiterpene hydrocarbons (18.33%), and alcohols (5.17%). The finding indicated that essential oils from different parts of Schinus molle have a promising potential on inhibiting activity of pathogenic microbes.
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